Abstract: Objective To study the effect of Xianling Gubao Capsule on bone strength of growing rats. Method Twenty one month old SD female rats of SPF grade were randomly divided into Control group and Xianling Gubao group, with 10 rats in each group. During the experiment, the whole body bone mineral density was measured every two weeks. After the sixth week, the Xianling Gubao group was significantly higher than the Control group, the samples were killed. The organs, femur, tibia and vertebrae were taken for pathological analysis, bone mineral density test, biomechanical test, micro CT analysis and serum biochemical indexes. Result During the experiment, the body mass of rats in each group showed an upward trend, there was no statistical significance between the two groups, and there were no obvious pathological changes in organs; After 6 weeks of administration, the BMD of whole body, femur and vertebra in Xianling Gubao group were significantly higher than those in the Control group (P < 0. 05 ) ; The maximum load and yield strength of femur in Xianling Gubao group were significantly higher than those in the Control group ( P< 0. 05) , and the maximum load of vertebra was also significantly higher than those in the Control group ( P < 0. 05) . The volume fraction, trabecular thickness and trabecular number of tibia in Xianling Gubao group were significantly higher than those in the Control group (P<0. 05) , and the separation degree of trabecular bone was significantly lower than that in the Control group ( P < 0. 05 ) ; Compared with the Control group, the content of serum OCN increased and the content of TRACP-5b decreased in Xianling Gubao group ( P < 0. 05 ) . Conclusion Xianling Gubao Capsule may improve the bone strength of growing rats by inhibiting bone absorption and promoting bone formation. It has the potential to prevent late osteoporosis by increasing the peak bone mass of growing rats.

Key words: Xianling Gubao capsule, growing rats, bone strength, peak bone mass

摘要： 目的 研究仙灵骨葆胶囊对生长期大鼠骨强度的影响。 方法 将 20 只 1 月龄 SPF 级 SD 雌性大鼠按随机数字表法分为对照组和仙灵骨葆组,每组 10 只。 仙灵骨葆组每天灌服 378 mg / kg 仙灵骨葆胶囊,对照组每天给予等体积蒸馏水。 实验期间每两周测一次全身骨密度,待第 6 周仙灵骨葆组显著高于对照组后安乐死取材。 取脏器、股骨、胫骨和椎骨进行病理学分析、骨密度检测、生物力学检测、micro CT 分析以及血清生化指标检测。 结果 实验期间各组大鼠体质量呈上升趋势,两组间比较并无统计学意义,脏器无明显病理学改变;给药 6 周后仙灵骨葆组全身骨密度、股骨骨密度和椎骨骨密度都显著高于对照组(P<0. 05) ;仙灵骨葆组股骨最大载荷和屈服强度显著高于对照组(P<0. 05) ,椎骨最大载荷也显著高于对照组( P<0. 05) ;仙灵骨葆组胫骨骨体积分数、骨小梁厚度和骨小梁数显著高于对照组(P<0. 05) ,骨小梁分离度显著低于对照组(P<0. 05) ;与对照组相比,仙灵骨葆组血清 OCN 含量上升、TRACP-5b 含量下降,差异具有统计学意义( P< 0. 05) 。 结论 仙灵骨葆胶囊可能通过抑制骨吸收与促进骨形成来提高生长期大鼠骨强度,具有通过提高生长期大鼠峰值骨量来预防后期骨质疏松的潜力。

关键词: 仙灵骨葆胶囊, 生长期大鼠, 骨强度, 峰值骨量